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rabbit polyclonal antibody against igfbp4  (Proteintech)


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    Proteintech rabbit polyclonal antibody against igfbp4
    Model genes expression validation; GSEA enrichment analysis, and correlation analysis based on <t>IGFBP4</t> . The relative expression levels of IGFBP4 , CTSC and APOE in A training and B testing sets. The relative expression levels of IGFBP4 , CTSC and APOE in C GSE100927 and D GSE20129. E UpSet plot showing shared pathways in the GSEA enrichment analysis of early and advanced plaques from GES28829 and GSE43292, which based on the expression of IGFBP4 . F The shared GSEA enrichment pathways based on the expression of IGFBP4 . The scatterplot displaying the correlation between IGFBP4 and G ACTA2 , H LUM , I TAGLN . (* p < 0.05; ** p < 0.01; *** p < 0.001****; p < 0.0001; ns, no statistical significance)
    Rabbit Polyclonal Antibody Against Igfbp4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+igfbp4/IGFBP4+Antibody/pmc11102212-101-5-14
    Average 93 stars, based on 12 article reviews
    rabbit polyclonal antibody against igfbp4 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis"

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis

    Journal: BMC Genomics

    doi: 10.1186/s12864-024-10379-y

    Model genes expression validation; GSEA enrichment analysis, and correlation analysis based on IGFBP4 . The relative expression levels of IGFBP4 , CTSC and APOE in A training and B testing sets. The relative expression levels of IGFBP4 , CTSC and APOE in C GSE100927 and D GSE20129. E UpSet plot showing shared pathways in the GSEA enrichment analysis of early and advanced plaques from GES28829 and GSE43292, which based on the expression of IGFBP4 . F The shared GSEA enrichment pathways based on the expression of IGFBP4 . The scatterplot displaying the correlation between IGFBP4 and G ACTA2 , H LUM , I TAGLN . (* p < 0.05; ** p < 0.01; *** p < 0.001****; p < 0.0001; ns, no statistical significance)
    Figure Legend Snippet: Model genes expression validation; GSEA enrichment analysis, and correlation analysis based on IGFBP4 . The relative expression levels of IGFBP4 , CTSC and APOE in A training and B testing sets. The relative expression levels of IGFBP4 , CTSC and APOE in C GSE100927 and D GSE20129. E UpSet plot showing shared pathways in the GSEA enrichment analysis of early and advanced plaques from GES28829 and GSE43292, which based on the expression of IGFBP4 . F The shared GSEA enrichment pathways based on the expression of IGFBP4 . The scatterplot displaying the correlation between IGFBP4 and G ACTA2 , H LUM , I TAGLN . (* p < 0.05; ** p < 0.01; *** p < 0.001****; p < 0.0001; ns, no statistical significance)

    Techniques Used: Expressing, Biomarker Discovery

    Pseudotime analysis between SMC2 and SMC5 clusters. A Nine stages of SMC differentiation. B The SMC2 and SMC5 cells using for pseudo-time analysis. C The temporal changing in cell differentiation. D The dynamic expression of IGFBP4 and marker genes along pseudotime
    Figure Legend Snippet: Pseudotime analysis between SMC2 and SMC5 clusters. A Nine stages of SMC differentiation. B The SMC2 and SMC5 cells using for pseudo-time analysis. C The temporal changing in cell differentiation. D The dynamic expression of IGFBP4 and marker genes along pseudotime

    Techniques Used: Cell Differentiation, Expressing, Marker

    Experimental validation of Igfbp4 expression. A Hematoxylin & eosin (HE) staining showing the macroscopic morphology and local magnification of vascular anatomical layers in rat carotid artery after balloon injury. (scale bar = 50 μm) B Localization of Igfbp4 expression in injury artery. HE Staining displaying the macroscopic and localized vascular landscapes of C normal artery, D early injured artery, and E advanced injured artery. (scale bar = 50 μm) F-G WB validating the protein expression levels of Igfbp4 in normal arteries, early injured arteries, and advanced injured arteries, using α-Tubulin as the housekeeping protein for normalization. H RT-qPCR experiment validating the mRNA expression levels of Igfbp4 in normal arteries, early injured arteries, and advanced injured arteries, using Gapdh as the housekeeping gene for normalization. (* p < 0.05; ** p < 0.01; *** p < 0.001)
    Figure Legend Snippet: Experimental validation of Igfbp4 expression. A Hematoxylin & eosin (HE) staining showing the macroscopic morphology and local magnification of vascular anatomical layers in rat carotid artery after balloon injury. (scale bar = 50 μm) B Localization of Igfbp4 expression in injury artery. HE Staining displaying the macroscopic and localized vascular landscapes of C normal artery, D early injured artery, and E advanced injured artery. (scale bar = 50 μm) F-G WB validating the protein expression levels of Igfbp4 in normal arteries, early injured arteries, and advanced injured arteries, using α-Tubulin as the housekeeping protein for normalization. H RT-qPCR experiment validating the mRNA expression levels of Igfbp4 in normal arteries, early injured arteries, and advanced injured arteries, using Gapdh as the housekeeping gene for normalization. (* p < 0.05; ** p < 0.01; *** p < 0.001)

    Techniques Used: Biomarker Discovery, Expressing, Staining, Quantitative RT-PCR

    The prediction of TFs and targeted compound. A Network diagram of TFs binding with IGFBP4 . B Box plot depicting the differences in TFs expression between early and advanced plaques. C The t-SNE plot displaying the expression level and localization of TFs in cell clusters. D The scatter plot showing the correlation between KLF15 and IGFBP4 . E The enlarged t-SNE plot for KLF15 . F The network diagram of compounds acting on KLF15 . (* p < 0.05; ** p < 0.01; *** p < 0.001)
    Figure Legend Snippet: The prediction of TFs and targeted compound. A Network diagram of TFs binding with IGFBP4 . B Box plot depicting the differences in TFs expression between early and advanced plaques. C The t-SNE plot displaying the expression level and localization of TFs in cell clusters. D The scatter plot showing the correlation between KLF15 and IGFBP4 . E The enlarged t-SNE plot for KLF15 . F The network diagram of compounds acting on KLF15 . (* p < 0.05; ** p < 0.01; *** p < 0.001)

    Techniques Used: Binding Assay, Expressing

    Related Articles

    Expressing:

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis.
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Biomarker Discovery:

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis.
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Cell Differentiation:

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis.
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Marker:

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis.
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Staining:

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis.
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Quantitative RT-PCR:

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis.
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Binding Assay:

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis.
    Article Snippet: Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.Immunofluorescence staining was employed to visualize the expression of Igfbp4 and Tagln in frozen sections of rat carotid artery tissue.. Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).. The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.The staining procedure involved the following steps: Sections were permeabilized using Triton X-100 (0.1%) for 30 min.



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    Model genes expression validation; GSEA enrichment analysis, and correlation analysis based on <t>IGFBP4</t> . The relative expression levels of IGFBP4 , CTSC and APOE in A training and B testing sets. The relative expression levels of IGFBP4 , CTSC and APOE in C GSE100927 and D GSE20129. E UpSet plot showing shared pathways in the GSEA enrichment analysis of early and advanced plaques from GES28829 and GSE43292, which based on the expression of IGFBP4 . F The shared GSEA enrichment pathways based on the expression of IGFBP4 . The scatterplot displaying the correlation between IGFBP4 and G ACTA2 , H LUM , I TAGLN . (* p < 0.05; ** p < 0.01; *** p < 0.001****; p < 0.0001; ns, no statistical significance)
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    Image Search Results


    Model genes expression validation; GSEA enrichment analysis, and correlation analysis based on IGFBP4 . The relative expression levels of IGFBP4 , CTSC and APOE in A training and B testing sets. The relative expression levels of IGFBP4 , CTSC and APOE in C GSE100927 and D GSE20129. E UpSet plot showing shared pathways in the GSEA enrichment analysis of early and advanced plaques from GES28829 and GSE43292, which based on the expression of IGFBP4 . F The shared GSEA enrichment pathways based on the expression of IGFBP4 . The scatterplot displaying the correlation between IGFBP4 and G ACTA2 , H LUM , I TAGLN . (* p < 0.05; ** p < 0.01; *** p < 0.001****; p < 0.0001; ns, no statistical significance)

    Journal: BMC Genomics

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis

    doi: 10.1186/s12864-024-10379-y

    Figure Lengend Snippet: Model genes expression validation; GSEA enrichment analysis, and correlation analysis based on IGFBP4 . The relative expression levels of IGFBP4 , CTSC and APOE in A training and B testing sets. The relative expression levels of IGFBP4 , CTSC and APOE in C GSE100927 and D GSE20129. E UpSet plot showing shared pathways in the GSEA enrichment analysis of early and advanced plaques from GES28829 and GSE43292, which based on the expression of IGFBP4 . F The shared GSEA enrichment pathways based on the expression of IGFBP4 . The scatterplot displaying the correlation between IGFBP4 and G ACTA2 , H LUM , I TAGLN . (* p < 0.05; ** p < 0.01; *** p < 0.001****; p < 0.0001; ns, no statistical significance)

    Article Snippet: Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).

    Techniques: Expressing, Biomarker Discovery

    Pseudotime analysis between SMC2 and SMC5 clusters. A Nine stages of SMC differentiation. B The SMC2 and SMC5 cells using for pseudo-time analysis. C The temporal changing in cell differentiation. D The dynamic expression of IGFBP4 and marker genes along pseudotime

    Journal: BMC Genomics

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis

    doi: 10.1186/s12864-024-10379-y

    Figure Lengend Snippet: Pseudotime analysis between SMC2 and SMC5 clusters. A Nine stages of SMC differentiation. B The SMC2 and SMC5 cells using for pseudo-time analysis. C The temporal changing in cell differentiation. D The dynamic expression of IGFBP4 and marker genes along pseudotime

    Article Snippet: Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).

    Techniques: Cell Differentiation, Expressing, Marker

    Experimental validation of Igfbp4 expression. A Hematoxylin & eosin (HE) staining showing the macroscopic morphology and local magnification of vascular anatomical layers in rat carotid artery after balloon injury. (scale bar = 50 μm) B Localization of Igfbp4 expression in injury artery. HE Staining displaying the macroscopic and localized vascular landscapes of C normal artery, D early injured artery, and E advanced injured artery. (scale bar = 50 μm) F-G WB validating the protein expression levels of Igfbp4 in normal arteries, early injured arteries, and advanced injured arteries, using α-Tubulin as the housekeeping protein for normalization. H RT-qPCR experiment validating the mRNA expression levels of Igfbp4 in normal arteries, early injured arteries, and advanced injured arteries, using Gapdh as the housekeeping gene for normalization. (* p < 0.05; ** p < 0.01; *** p < 0.001)

    Journal: BMC Genomics

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis

    doi: 10.1186/s12864-024-10379-y

    Figure Lengend Snippet: Experimental validation of Igfbp4 expression. A Hematoxylin & eosin (HE) staining showing the macroscopic morphology and local magnification of vascular anatomical layers in rat carotid artery after balloon injury. (scale bar = 50 μm) B Localization of Igfbp4 expression in injury artery. HE Staining displaying the macroscopic and localized vascular landscapes of C normal artery, D early injured artery, and E advanced injured artery. (scale bar = 50 μm) F-G WB validating the protein expression levels of Igfbp4 in normal arteries, early injured arteries, and advanced injured arteries, using α-Tubulin as the housekeeping protein for normalization. H RT-qPCR experiment validating the mRNA expression levels of Igfbp4 in normal arteries, early injured arteries, and advanced injured arteries, using Gapdh as the housekeeping gene for normalization. (* p < 0.05; ** p < 0.01; *** p < 0.001)

    Article Snippet: Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).

    Techniques: Biomarker Discovery, Expressing, Staining, Quantitative RT-PCR

    The prediction of TFs and targeted compound. A Network diagram of TFs binding with IGFBP4 . B Box plot depicting the differences in TFs expression between early and advanced plaques. C The t-SNE plot displaying the expression level and localization of TFs in cell clusters. D The scatter plot showing the correlation between KLF15 and IGFBP4 . E The enlarged t-SNE plot for KLF15 . F The network diagram of compounds acting on KLF15 . (* p < 0.05; ** p < 0.01; *** p < 0.001)

    Journal: BMC Genomics

    Article Title: Deciphering smooth muscle cell heterogeneity in atherosclerotic plaques and constructing model: a multi-omics approach with focus on KLF15/IGFBP4 axis

    doi: 10.1186/s12864-024-10379-y

    Figure Lengend Snippet: The prediction of TFs and targeted compound. A Network diagram of TFs binding with IGFBP4 . B Box plot depicting the differences in TFs expression between early and advanced plaques. C The t-SNE plot displaying the expression level and localization of TFs in cell clusters. D The scatter plot showing the correlation between KLF15 and IGFBP4 . E The enlarged t-SNE plot for KLF15 . F The network diagram of compounds acting on KLF15 . (* p < 0.05; ** p < 0.01; *** p < 0.001)

    Article Snippet: Primary antibodies used included a rabbit polyclonal antibody against Igfbp4 (1:20 Cat No: 18500-1-AP, Proteintech) and a mouse monoclonal antibody against Tagln (1:500 Cat No: 60213-1-Ig, Proteintech).

    Techniques: Binding Assay, Expressing